Methods of treating fibrosing diseases by induction of immune tolerance

ABSTRACT

The present invention has demonstrated for the first time that orally administered type I collagen (CI) induced tolerance to CI in patients suffering from systemic sclerosis (SSc) and ameliorated clinical manifestations of the disease. Accordingly, the present invention provides methods of treating a fibrosing disease by oral administration of a tissue protein, for example, collagen, derived from the tissue undergoing fibrosis.

CROSS REFERENCE TO RELATED APPLICATION

This application is a continuation of U.S. patent application Ser. No.12/749,141, filed Mar. 29, 2010, which is a continuation of U.S. patentapplication Ser. No. 11/600,525, filed Nov. 16, 2006, now U.S. Pat. No.7,718,765, which claims priority to U.S. Provisional Application No.60/737,194, filed on Nov. 16, 2005. The contents of each areincorporated herein.

FIELD OF THE INVENTION

This invention generally relates to treatment of fibrosing diseases. Inparticular, the present invention relates to treatment of fibrosingdiseases by induction of immune tolerance.

BACKGROUND OF THE INVENTION

Acquired fibrosing diseases in humans have several common features.Tissue fibrosis is preceded by injury to and/or inflammation of thenormal tissue. Infiltrations of the tissue by T cells and monocytes arepresent in the early phases of fibrosis development.

Systemic sclerosis (SSc, scleroderma) is a prototypic systemic fibrosingdisease associated with increased accumulation of collagen type I, III,IV, VI, VII, XVI, XVIII. Cellular and/or humoral immunity to types I,III and IV have been described in patients with SSc. The disease mostcharacteristically involves the skin which becomes thick and tightlybound to underlying structures. The internal organs commonly involvedare gastrointestinal tract, lungs, kidneys, and heart.

T lymphocytes via synthesis of cytokines of different types can modulatethe functions of fibroblasts and monocytes/macrophages as well as avariety of other target cells. With regards to fibrosis, the productionof fibrogenic cytokines by T cells such as IL-4, TGF-β1 and β2, candirectly stimulate synthesis of collagen by fibroblasts in culture. Tcells by secreting interferon (IFN) gamma can activate macrophages,which in turn can synthesize several fibrogenic cytokines includingplatelet derived growth factor, TGF-β1 and β2 which in turn canstimulate fibroblasts to increase synthesis of collagen.

SUMMARY OF THE INVENTION

The present invention provides methods for treating a fibrosing diseaseby oral administration of a tissue protein derived from the tissueundergoing fibrosis.

The fibrosing diseases that can be treated in accordance with thepresent invention include, but are not limited to, scleroderma (SSc),skin fibrosis, liver cirrhosis, renal fibrosis, lung fibrosis, heartfibrosis, gastrointestinal fibrosis and vascular fibrosis.

In one embodiment, the present methods are utilized to treat a patientsuffering from a fibrosing disease for at least 3 years, preferably, forat least 5 years.

In another embodiment, a fibrosing disease is treated by oraladministration of a collagen derived from the tissue(s) undergoingfibrosis. Depending upon the tissue type, different types of collagenmay be employed in the treatment. Collagen can be prepared from thetissue undergoing fibrosis in a human patient, or from the correspondingtissue of an animal, such as an avian species or a mammal.Alternatively, chemically synthesized or recombinantly produced collagencan be employed. A fragment or a mixture of fragments of collagen canalso be employed according to the present invention.

In a preferred embodiment, collagen or fragments of collagen areprovided to a patient by oral administration at about 500 μg/day forabout 12 months.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 depicts boxplots of changes in MRSS at different time points indifferent SSc patient subgroups.

FIG. 2 correlates percentages of SSc patients versus percentages ofimprovement in MRSS at 12 month.

FIG. 3 correlates percentages of SSc patients versus percentages ofimprovement in MRSS at 15 month.

FIG. 4 is a graphical representation of α1(I) and α2(I) cleaved withcyanogen bromide (CNBr). The solid triangles represent the location ofterminal determinants, and the hollow triangles represent the locationof central non helical determinants. CNBr cleavage of α1(I) yields eightCB fragments: CB0, CB1, CB2, CB4, CB5, CB8, CB3, CB7 and CB6. CNBrcleavage of α2(I) yields six CB fragments: CB1, CB0, CB4, CB2, CB3 andCB5. The amino acid residues of each CB peptide are shown in Table III.

DETAILED DESCRIPTION OF THE INVENTION

The present invention has demonstrated for the first time that orallyadministered type I collagen (CI) induced tolerance to CI in patientssuffering from systemic sclerosis (SSc) and ameliorated clinicalmanifestations of the disease.

SSc is a prototypic systemic fibrosing disease associated with anincreased accumulation of extracellular matrix proteins such ascollagen. Without intending to be bound by any particular theory, it isbelieved that oral administration of a tissue protein (such as collagen)present at the tissue site undergoing fibrosis where T cells are beingactivated by various stimuli, can down-regulate T cells. Consequently, Tcells are inhibited from secreting fibrogenic cytokines and cytokinesthat activate monocytes/macrophages, which cytokines would otherwisestimulate fibroblasts at the tissue site to produce extracellular matrixproteins such as collagen.

Accordingly, the present invention provides methods of treating afibrosing disease by oral administration of a tissue protein derivedfrom the tissue undergoing fibrosis.

The fibrosing diseases that can be treated with the present methodsinclude, but are not limited to, SSc, skin fibrosis, liver cirrhosis,renal fibrosis, lung fibrosis, heart fibrosis (as occurs, for example,in congestive heart failure), gastrointestinal fibrosis and vascularfibrosis as occurs in atherosclerosis. The methods of the presentinvention can treat these fibrosing diseases regardless of the cause ofthe disease.

In a specific embodiment, the present methods are utilized to treat apatient suffering from a fibrosing disease for at least 3 years,preferably, for at least 5 years.

According to the present invention, a fibrosing disease can be treatedby oral administration of a collagen derived from the tissue(s)undergoing fibrosis. For example, SSc is known to associate withexcessive accumulation of type I collagen, and therefore type I collagenor a fragment thereof is orally administered to patients suffering SSc.Liver cirrhosis, lung fibrosis, and interstitial collagen disease areassociated with increased accumulation of type I, III, and V collagen,respectively. Therefore, type I, III and V collagens or a fragment(s)thereof are orally administered to patients suffering from livercirrhosis, lung fibrosis, and interstitial collagen disease,respectively. Small synthetic peptides from collagen may also inducetolerance when given nasally, for example, by nose drops or nose spray,or inhaled by aerosolization.

Collagen can be prepared and extracted from the tissue undergoingfibrosis in a human patient, or from the corresponding tissue(s) of ananimal, such as an avian species (e.g., domestic chickens) or a mammal(e.g., bovine or porcine). Alternatively, chemically synthesized orrecombinantly produced collagen can be employed. Moreover, a fragment ora mixture of fragments of collagen can also be employed according to thepresent invention. For example, peptides derived by cleavage of type Icollagen with CNBr can be employed in treating a patient suffering fromSSc.

Collagen or fragments of collagen can be provided to a patient by oraladministration at about 200-1000 μg/day, preferably about 400-600μg/day, and more preferably at about 500 μg/day. The treatment cancontinue for at least six months, preferably 12 months or longer, oruntil the clinical manifestations of the disease are reduced orameliorated.

The present invention is further illustrated by the following examples.

EXAMPLE 1

To determine whether orally administered bovine type I collagen (Cl) atdoses of 500 μg/day ameliorates clinical manifestations of systemicsclerosis (SSc), a multicenter double blind placebo-controlled study wasconducted.

Patients were screened based on the following criteria in order to beincluded in the study:

-   -   Male or female of at least 18 years old;    -   Clinically diagnosed to have diffuse SSc (by ACR criteria 1980)        for 3 years or less (early phase), or between 4 and 10 years        (late phase);    -   Stable skin involvement by history or physical examination        during the 6 months preceding enrollment; and    -   Stable modified Rodnan skin score (MRSS) 1 month preceding        enrollment: stable MRSS≧16 at screening and stable MRSS at        randomization (baseline) as follows:

Allowable MRSS MRSS at screen at randomization (baseline)    16 up to 2017-20 16-24 21-25 ±4 26-30 ±5 ≧31 ±7

168 patients who met the foregoing criteria were stratified andrandomized to receive daily placebo [2 ml 0.1M acetic acid (HAc)] or 500μg bovine CI for 12 months. MRSS was measured as a primary clinicaloutcome variable at baseline and after 4, 8, 12, and 15 months.Scleroderma Health Assessment Questionnaire (SHAM), Short Form 36(SF-36) questionnaire, Physician's Global Assessment, Patient's GlobalAssessment, blood pressure, weight and serum creatinine were determinedas secondary clinical outcome measures at baseline and after 4, 8, 12,and 15 months. Patients had FVC and DLCO measured no earlier than 5weeks before baseline, and 12 months as secondary clinical outcomeparameters. A pre screening visit was also required for patients takingany exclusionary drugs/treatments.

FIG. 1 summarizes the changes in MRSS at month 4 (blue), month 8 (red),month 12 (green) and month 15 (orange) from baseline and broken down bythe four subgroups. Each boxplot describes the distribution of thechange in MRSS in each group and at each time point; the upper edge isthe 75% percentile; the lower edge is the 25%; and the line inside thebox represents the median change in MRSS. Outlying values are presentedby whiskers from the box. The results indicate that there was nostatistical difference in the mean change between the CI-treated groupand the placebo group at 12 month. Similar conclusions applied to theother clinical and laboratory parameters as well (see Table 1 and Table2). However, at 15 month, there was a very noticeable change in MRSS:7.9 in the late phase patients treated with CI (the “late collagen”group) and 2.9 in the late phase patients in the placebo (“lateplacebo”) group. As shown in FIG. 1, at 15 month, the median value inthe orange box for the late phase patient group treated with collagen isclearly substantially lower than the median values in the other orangeboxes, and in fact is also the lowest for all the boxes. This means thatpatients in the late phase subgroup treated with CI experienced thegreatest improvement in MRSS. The p-value of the mean difference in MRSSbetween treatment groups for late phase patients is 0.0063; all othertests are not significant at the 0.05 level. It is noted that thevariable MRSS by itself is not normally distributed, but the change inMRSS at 12 or 15 month from baseline is normally distributed. Hence, thep-value was obtained from the t-test. A non-parametric test was alsoused to ascertain change in MRSS between the treated and placebo group,namely the rank-sum test, and the p-value was similar.

When changes in MRSS were dichotomized and the percentage of patientswho had skin improvement in MRSS was determined, two graphs wereobtained (FIG. 2 and FIG. 3). Each graph plots the percentage of thecohort in each of the four subgroups who experienced different degreesof improvement in MRSS. For instance, in the first plot, almost 50% ofpatients in the late collagen group had a reduction of 20% in MRSS at 12month. In contrast, only about 19% experienced a similar improvement inthe early collagen group. Both plots clearly show that the late phasepatients benefited most from the collagen treatment compared with theother subgroups. Among the collagen group, the Chi-squared testconfirmed that at 15 months, a significantly higher proportion of thelate phase patients had at least a 25% improvement in MRSS compared withthe early phase patients.

In sum, the foregoing study shows that orally administered bovine CI at500 μg/day for 12 months was found to significantly decrease the MRSS atMonth 15 of the study in patients with disease duration of ≧4 to 10years, indicating a delayed effect of the oral collagen treatment onskin fibrosis. There were no discernable effects of oral CI in thisstudy on PFTs or HAQ, and no adverse events that could be attributableto the CI treatment. The delayed effect of the oral collagen treatmentis consistent with the notion that it takes some time for fibroblasts to“wind down” once the T cell stimuli are neutralized. These results alsosuggest that T cells provide a major source of fibrogenic signals onlyin late phase patients.

EXAMPLE 2

This Example describes experiments conducted to determine whether theoral CI treatment at 500 μg/day induced tolerance to CI in the patientsenrolled in the study described in Example 1.

Serum and PBMC were obtained from patients before and after the 12 monthtreatment with oral bovine CI, or at drop-out greater than or equal to 3months to less than or equal to 11 months. The PBMC were cultured withor without bovine α1(I) chain, bovine α2(I) chain, native bovine CI, orCB (CNBr) peptides of α1(I) or α2(I). CB peptides were isolated bycleavage of bovine or human α1(I) and α2(I) with CNBr (illustrated inFIG. 4 and Table 3) and purification by ion exchange chromatography.Purified CB peptides of α1(I) and α2(I) as well as unseparated CBpeptides of α1(I) and α2(I) were used in the culture of PBMC from SScpatients at baseline before administration of CI or placebo and at 12months. The PBMC supernatants were analyzed by ELISA for IFNγ and IL-10,at 0 and 12 months. Decreases in IFNγ or increases in IL-10 productionby a chain-stimulated PBMC after oral CI were determined as the primaryimmunology outcome variable. The results are summarized in Tables 4-9.

As can be seen from Tables 5-6, significant decreases were observed inthe production of IFNγ by PMBC to α1(I) CB peptide mixture and to α1(I)CB7 in the Total and Early Disease Phase patient population treated withoral CI for 12 months. Additionally, significant increases were observedin the IL-10 production by PBMC cultured with human α2(I) and α1(I) CB7in the Total and Late Phase patient population (Tables 7-8). Theseresults suggest that oral Bovine CI is potentially efficacious intreating patients with diffuse SSc of ≧4 years duration apparently bymodulating THI/TH2 production. Upregulation of antigen-specific IL-10production suggests that tolerance was induced to CI in LD patients.

For the total SSc population, there were inverse correlations betweendisease duration and IL-10 production by the following: α1(I) CB3(p=−0.0059. N=153); α1(I) CB7 (p=−0.0335, N=150); human α1(I)(p=−0.0166, N=152); and α2(I) CB Mixture (p=−0.0032. N=154).

For Early patients, there was an inverse correlation between MRSS andIFNγ production to α2(I) CB2 (p=−0.026, N=94).

For the total SSc population, there was an inverse correlation betweenSF-36 and IFNγ production to α1(I) CB4 (p=−0.0448, N=143). For Latepatients, there were inverse correlations between SF-36 and IFNγproduction to α1(I) CB4 and PHA (p=−0.0364, N=57; p=−0.028, N=58,respectively).

For the total diffuse SSc population, there were direct correlationsbetween FVC and IL-10 production by PBMC cultured with α1(I) CB4 andhuman α2(I) (p=0.0122, N=152; p=0.0072, N=94, respectively).

For Early patients, there was a direct correlation between FVC and IL-10production to human α2(I) (p=0.0062, N=94).

For Early Patients, there was an inverse correlation between FEV1 andIL-10 production to α2(I) CB4 and α1(I) CB Mixture (p=−0.0067, N=92;p=−0.0041, N=94, respectively). For the total diffuse SSc population,there was an inverse correlation between FEV1 and IL-10 production toα1(I) CB Mixture (p=0.0241, N=154).

In the Early patients, there was a direct correlation between DLCO andIFNγ production to α1(I) CB7 (p=0.0367, N=90). In the Late patients,there was a direct correlation between DLCO and IFNγ production to α2(I)CB2 (p=0.0383, N=59).

In sum, the immune response studies conducted by culturing PBMC from thepatients with CI and CI-derived peptides showed that, in general,greater IFNγ and IL-10 production by cultured PBMC occurred in patientswith Early Phase diseases<<4 years duration). IFNγ production to theantigen C. albicans was absent in both early and late phase patients,suggesting impaired Th1 responsiveness to common environmental antigens.Native Bovine CI elicited significant increases in IFNγ and IL-10production in both early and late phase patients. Specific CI CBpeptides that failed to elicit IFNγ or IL-10 production in late phasepatients included α1(I) CB2, 4, 5 and 7, and α2(I) CB2, 3 and 3-5. Thestrongest consistent IFNγ and IL-10 response in both early and latephase patients was observed with α1(I) CB8, α1(I) CB6, α2(I) CB4,indicating these portions of α1(I) and α2(I) contain epitopes thatelicit T cell responses throughout the duration of the disease in themajority of patients with diffuse SSc. Correlations between specificPBMC IFNγ or IL-10 responses to CI and CI derived peptides suggest thatsubsets of patients might exist in which the particular cytokineresponse to specific CI epitopes might influence disease expression.

TABLE 1 Scleroderma HAQ Changes Between Baseline and Months 12 and 15Month 12 Month 15 p p (n) Mean ± SEM value (n) Mean ± SEM value Total(56) −0.028 ± 0.061   NS (56) 0.0222 ± 0.061  NS Placebo Total (46)0.114 ± 0.078 (39) 0.674 ± 0.084 C1 Early (27) −0.022 ± 0.093   NS (28)0.009 ± 0.098 NS Placebo Early (30) 0.125 ± 0.097 (24) −0.010 ± 0.096  C1 Late (30) −0.0337 ± 0.081    NS (28) 0.054 ± 0.076 NS Placebo Late C1(16) 0.094 ± 0.134 (15) 0.191 ± 0.155

TABLE 2 PFT Changes Between Baseline and Month 12 (n) FEV₁ (n) FVC (n)DL_(co) p p p Mean ± SEM value Mean ± SEM value Mean ± SEM value TotalPlacebo (61) −0.46 ± 1.67 NS (60) −0.68 ± 1.20 NS (59) −2.66 ± 2.23 NSTotal CI (47) −2.02 ± 3.05 (40) −4.54 ± 2.41 (47) −5.74 ± 2.58 EarlyPlacebo (30) −0.17 ± 3.18 NS (29) −0.83 ± 2.11 NS (29) −1.76 ± 3.21 NSEarly CI (30) −2.07 ± 4.74 (31) −5.42 ± 3.66 (31) −4.81 ± 3.77 LatePlacebo (31) −0.74 ± 1.23 NS (31) −0.55 ± 1.25 NS (30) −3.53 ± 2.59 NSLate CI (17) −1.94 ± 1.45 (17) −2.94 ± 1.42 (16) −7.51 ± 3.99

TABLE III Amino Acid Residues Contained in Human CI CB Peptides Residue# CB Peptide Number of Amino Acid Residues Human α1(I) CB Peptides 1-3CB1 3  4-39 CB2 36 40-86 CB4 47  87-123 CB5 36 124-402 CB8 279 403-551CB3 149 552-842 CB7 291  843-1014 CB6 172 Human α2(I) CB Peptides 1-3CB0 3 4-6 CB1 3  7-327 CB4 321 328-357 CB2 30 358-695 CB3 338  696-1014CB5 319

TABLE 4 IFNγ Production at Baseline and 12 Months (n) PBS (n) PHA (n)Cand (n) H α1 (I) (n) Hα2 (I) BCI Total Placebo Baseline (58) 389 ± 71(57) 2837 ± (50) 497 ± 113 (55) 876 ± 139 (55) 766 ± 132 (57) 728 ± 116190 Month 12 (46) 734 ± 111 (58) 671 ± 133 (54) 769 ± 107 (54) 685 ± 112(58) 970 ± 150 Total CI Baseline (46) 407 ± 71 (46) 2844 ± (38) 840 ±194 (44) 950 ± 176 (44) 848 ± 163 (46) 638 ± 118 233 Month 12 (46) 508 ±79 (46) 2178 ± (45) 539 ± 140 (45) 767 ± 113 (45) 749 ± 103 (46) 655 ±126 p value 0.184 0.046 0.888 0.544 0.293 Early Placebo Baseline (28)239 ± 57 (27) 2955 ± (24) 339 ± 124 (26) 627 ± 147 (26) 516 ± 119 (27)714 ± 187 250 Month 12 (28) 648 ± 131 (28) 487 ± 115 (26) 571 ± 82 (26)536 ± 92 (28) 795 ± 194 Early CI Baseline (30) 324 ± 81 (30) 2982 ± 28(25) 989 ± 283 (29) 1190 ± (29) 1042 ± (30) 672 ± 166 253 236 Month 12(30) 544 ± 108 (30) 2062 ± (29) 627 ± 211 (29) 692 ± 122 (29) 684 ± 116(30) 709 ± 177 291 p value 0.371 0.246 0.798 Late Placebo Baseline (30)528 ± 123 (30) 2731 ± (26) 644 ± 183 (29) 1099 ± (29) 990 ± 220 (30) 740± 146 286 222 Month 12 (30) 815 ± 178 (30) 842 ± 231 (28) 823 ± 196 (28)795 ± 195 Late CI Baseline (16) 563 ± 133 (16) 2585 ± (13) 554 ± 150(15) 486 ± 102 (15) 473 ± 103 (16) 573 ± 143 352 Month 12 (16) 439 ± 103(16) 2397 ± (16) 378 ± 90 (16) 902 ± 230 (16) 866 ± 203 (16) 554 ± 147 pvalue 0.153 0.245 0.106 0.061 0.240

TABLE 5 IFNγ Production by SSc PBMC Cultured with Bovine α1(I) CBPeptides at Baseline and 12 Months α1(I) CB Peptides (n) CB Mix (n) CB2(n) CB4 (n) CB5 (n) CB8 (n) CB3 (n) CB7 (n) CB6 Total Placebo Base- (58)812 ± 160 (56) 640 ± 97 (56) 777 ± 159 (56) 611 ± 126 (56) 872 ± 147(57) 955 ± 161 (55) 651 ± 121 (55) 856 ± 151 line Month (57) 898 ± 138(55) 743 ± (55) 814 ± 122 (56) 750 ± 123 (57) 832 ± 103 (55) 1209 ± 172(57) 822 ± 116 (56) 1044 ± 142 12 Total CI Base- (45) 1006 ± (45) 620 ±(46) 760 ± 171 (46) 856 ± 183 (46) 986 ± 167 (46) 963 ± 183 (46) 783 ±135 (46) 1001 ± 187 line 173 142 Month (45) 683 ± 114 (46) 487 ± 80 (46)862 ± 173 (46) 713 ± 125 (46) 808 ± 144 (46) 943 ± 179 (46) 591 ± 97(46) 1004 ± 176 12 p value 0.260 0.565 0.464 0.156 0.793 0.034 0.294Early Placebo Base- (28) 769 ± 240 (27) 656 ± (27) 643 ± 162 (27) 534 ±152 (26) 659 ± 197 (28) 876 ± 207 (26) 492 ± 151 (26) 648 ± 190 line 123Month (28) 766 ± 170 (26) 643 ± 121 (27) 601 ± 145 (27) 624 ± 121 (27)1061 ± 224 (27) 557 ± 89 (27) 925 ± 180 12 Early CI Base- (29) 1105 ±(30) 501 ± (30) 856 ± 226 (30) 969 ± 253 (30) 1124 ± 234 (30) 1148 ± 241(30) 903 ± 176 (30) 1215 ± 265 line 249 136 Month (29) 650 ± 138 (30)445 ± (30) 737 ± 182 (30) 792 ± 184 (30) 869 ± 199 (30) 1053 ± 256 (30)609 ± 136 (30) 1040 ± 227 12 p value 0.060 0.408 0.421 0.088 0.935 0.0060.0632 Late Placebo Base- (30) 851 ± 217 (29) 626 ± (29) 903 ± 269 (29)682 ± 200 (30) 1056 ± 213 (29) 1031 ± 247 (29) 793 ± 183 (29) 1042 ± 228line 149 Month (29) 1025 ± (29) 967 ± 203 (29) 889 ± (30) 1020 ± 155(28) 1352 ± 261 (30) 1060 ± (29) 1154 ± 217 12 Late CI Base- (16) 877 ±252 (15) 856 ± (16) 579 ± 255 (16) 644 ± 227 (16) 727 ± 189 (16) 615 ±258 (16) 559 ± 199 (16) 601 ± 174 line 328 Month (16) 743 ± 206 (16) 564± (16) 1097 ± (16) 565 ± 97 (16) 693 ± 188 (16) 739 ± 185 (16) 559 ±(16) 937 ± 280 12 364 123 p value 0.265 0.874 0.764 0.980 0.479

TABLE 6 IFN γ Production by SSc PBMC Cultured with Bovine α2(I) CBPeptides at Baseline and 12 Months α2 CB Peptides (n) CB Mix (n) CB4 (n)CB2 (n) CB3 (n) CB 3-5 Total Placebo Baseline (58) 886 ± 165 (56) 854 ±147 (58) 584 ± 117 (56) 774 ± 137 (58) 795 ± 146 Month 12 (57) 902 ± 135(56) 801 ± 110 (57) 647 ± 109 (55) 873 ± 128 (57) 872 ± 121 Total CIBaseline (45) 1006 ± 187  (46) 842 ± 156 (46) 551 ± 110 (45) 1022 ± 196 (46) 830 ± 172 Month 12 (45) 619 ± 102 (45) 690 ± 130 (46) 424 ± 69 (45) 805 ± 147 (46) 696 ± 111 p value  0.0185 0.300 0.139 0.440 0.372Early Placebo Baseline (28) 763 ± 228 (26) 581 ± 161 (28) 558 ± 194 (26)556 ± 157 (28) 711 ± 222 Month 12 (28) 817 ± 76  (27) 643 ± 101 (27) 496± 101 (27) 776 ± 153 (27) 739 ± 142 Early CI Baseline (29) 1105 ± 249 (30) 1008 ± 227  (30) 489 ± 110 (30) 1290 ± 276  (30) 1022 ± 245  Month12 (29) 606 ± 126 (29) 669 ± 176 (30) 443 ± 94  (29) 879 ± 205 (30) 761± 158 p value 0.011 0.078 0.153 0.083 0.087 Late Placebo Baseline (30)1001 ± 241  (30) 1091 ± 230  (30) 609 ± 138 (30) 962 ± 213 (30) 874 ±194 Month 12 (29) 984 ± 206 (29) 948 ± 189 (30) 783 ± 184 (28) 966 ± 205(30) 991 ± 189 Late CI Baseline (16) 827 ± 275 (16) 532 ± 117 (16) 667 ±244 (15) 486 ± 120 (16) 470 ± 159 Month 12 (16) 644 ± 178 (16) 730 ± 187(16) 388 ± 96  (16) 673 ± 189 (16) 578 ± 127 p value 0.507 0.687 0.4750.359 0.661

TABLE 7 IL-10 Production by SSc PBMC at Baseline and 12 Months (n) PBS(n) PHA (n) Cand (n) BCI (n) Hα1(I) (n) Hα2 (I) Total Placebo Baseline(65) 382 ± 93 (64) 1500 ± 177 (52) 168 ± 40 (64) 537 ± 83 (65) 827 ± 99(65) 641 ± 85 Month 12 (65) 277 ± 36 (63) 1131 ± 135 (64) 195 ± 23 (65)240 ± 25 (61) 579 ± 85 (61) 433 ± 47 Total CI Baseline (51) 247 ± 48(51) 1263 ± 219 (41) 118 ± 24 (51) 398 ± 91 (49) 604 ± 87 (49) 425 ± 71Month 12 (51) 293 ± 43 (51) 1438 ± 219 (50) 260 ± 50 (51) 256 ± 37 (49)536 ± 66 (49) 466 ± 66 p value 0.565 0.172 0.778 0.234 0.093 0.052 EarlyPlacebo Baseline (34) 308 ± 77 (33) 1656 ± 258 (28) 205 ± 69 (33) 577 ±128 (34) 984 ± 155 (34) 700 ± 135 Month 12 (34) 328 ± 59 (32) 1180 ± 189(33) 212 ± 33 (34) 264 ± 38 (32) 578 ± 109 (32) 463 ± 71 Early CIBaseline (34) 271 ± 64 (34) 1314 ± 264 (27) 136 ± 34 (34) 478 ± 127 (33)665 ± 112 (33) 462 ± 96 Month 12 (34) 281 ± 43 (34) 1290 ± 119 (33) 235± 57 (34) 239 ± 42 (33) 495 ± 78 (33) 400 ± 59 p value 0.922 0.541 0.9660.826 0.314 0.376 Late Placebo Baseline (31) 463 ± 175 (31) 1334 ± 242(24) 125 ± 30 (31) 493 ± 106 (31) 655 ± 113 (31) 577 ± 99 Month 12 (31)220 ± 37 (31) 1082 ± 194 (31) 177 ± 32 (31) 213 ± 32 (29) 581 ± 136 (29)400 ± 62 Late CI Baseline 75(17) 199 ± 64 (17) 1161 ± 403 (14) 83 ± 24(17) 239 ± 96 (16) 479 ± 131 (16) 348 ± 90 Month 12 (17) 319 ± 98 (17)1734 ± 476 (17) 310 ± 100 (17) 289 ± 75 (16) 621 ± 123 (16) 602 ± 159 pvalue 0.457 0.185 0.868 0.070 0.122 0.039

TABLE 8 IL-10 Production by SSc PBMC Cultured with Bovine α1(I) CBPeptides at Baseline and 12 months α1(I) CB Peptides (n) α1 CB Mix (n)CB2 (n) CB4 (n)CB5 (n) CB8 (n) CB3 (n) CB7 (n) CB6 Total Placebo Base-(64) 714 ± 97 (62) 297 ± 53 (62) 785 ± 180 10(62) 426 ± 61 (64) 881 ±106 (63) 696 ± 98 (63) 476 ± 61 (62) 754 ± 99 line Month (64) 491 ± 55(62) 341 ± 47 (62) 519 ± 61 (63) 425 ± 52 (64) 619 ± 67 (62) 713 ± 79(64) 461 ± 52 (63) 746 ± 89 12 Total CI Base- (50) 608 ± 108 (49) 244 ±44 (50) 727 ± 208 (49) 357 ± 61 (49) 656 ± 98 (50) 633 ± 103 (49) 377 ±63 (49) 867 ± 128 line Month (50) 515 ± 86 (50) 362 ± 53 (50) 852 ± 176(50) 452 ± 58 (51) 714 ± 88 (50) 810 ± 97 (51) 531 ± 55 (50) 750 ± 88 12p value 0.508 0.505 0.180 0.360 0.129 0.159 0.046 0.638 Early PlaceboBase- (33) 858 ± 163 (32) 361 ± 94 (32) 1134 ± 307 (32) 502 ± 102 (33)1069 ± 177 (33) 904 ± 173 (33) 562 ± 106 (32) 986 ± 161 line Month (34)572 ± 92 (33) 392 ± 78 (32) 503 ± 83 (33) 495 ± 80 (33) 701 ± 112 (33)795 ± 116 (33) 560 ± 83 (33) 768 ± 114 12 Early CI Base- (33) 1699 ±(33) 244 ± 45 (33) 756 ± 241 (33) 366 ± 76 (33) 667 ± 133 (33) 712 ± 149(33) 430 ± 87 (33) 863 ± 150 line 157 Month (33) 413 ± 61 (34) 351 ± 71(34) 789 ± 200 (34) 381 ± 53 (34) 615 ± 74 (34) 799 ± 118 (34) 480 ± 57(34) 695 ± 82 12 p value 0.969 0.957 0.082 0.943 0.126 0.362 0.568 0.753Late Placebo Base- (31) 560 ± 95 (30) 228 ± 43 (30) 413 ± 153 (30) 344 ±63 (31) 681 ± 102 (30) 467 ± 57 (30) 382 ± 51 (30) 505 ± 94 line Month(30) 399 ± 50 (29) 283 ± 46 (30) 537 ± 92 (30) 347 ± 63 (31) 532 ± 66(29) 621 ± 104 (31) 356 ± 56 (30) 721 ± 140 12 Late CI Base- (17) 431 ±80 (16) 224 ± 102 (17) 671 ± 403 (16) 340 ± 106 (16) 633 ± 126 (17) 479± 79 (16) 269 ± 70 (16) 880 ± 246 line Month (17) 714 ± (16) 386 ± 73(16) 987 ± 357 (16) 605 ± 138 (17) 911 ± 213 (16) 833 ± 174 (17) 633 ±119 (16) 866 ± 214 12 219 p value 0.232 0.293 0.545 0.141 0.530 0.1880.010 0.304

TABLE 9 IL-10 Production by SSc PBMC Cultured with α2 (I) CP Peptides atBaseline and 12 Months α2 (I) CB Peptides (n) CB Mix (n) CB4 (n) CB2 (n)CB3 (n) CB3-5 Total Placebo Baseline (64) 700 ± 87 (62) 910 ± 114 (64)331 ± 57 (63) 904 ± 137 (64) 866 ± 133 Month 12 (64) 466 ± 49 (61) 596 ±68  (65) 349 ± 42 (61) 660 ± 77  (65) 612 ± 73  Total CI Baseline  (50)719 ± 114 (50) 725 ± 108 (50) 225 ± 33 (48) 713 ± 106 (50) 840 ± 121Month 12 (50) 510 ± 64 (49) 583 ± 75  (50) 317 ± 35 (50) 707 ± 83  (49)657 ± 92  p value 0.292 0.356 0.772 0.283 0.909 Early Placebo Baseline (33) 804 ± 147 (31) 1082 ± 179   (33) 422 ± 102 (32) 1107 ± 244  (33)1044 ± 230  Month 12 (34) 502 ± 79 (32) 684 ± 118 (34) 386 ± 63 (32) 772± 130 (34) 708 ± 117 Early CI Baseline  (33) 844 ± 164 (33) 748 ± 149(33) 216 ± 34 (33) 754 ± 132 (33) 905 ± 151 Month 12 (33) 475 ± 61 (33)549 ± 85  (34) 312 ± 42 (33) 649 ± 87  (33) 670 ± 115 p value 0.8650.367 0.837 0.711 0.600 Late Placebo Baseline (31) 589 ± 87 (31) 737 ±135 (31) 234 ± 41 (31) 694 ± 111 (31) 676 ± 119 Month 12 (30) 425 ± 53(29) 500 ± 58  (31) 308 ± 53 (29) 536 ± 73  (31) 506 ± 80  Late CIBaseline (17) 478 ± 85 (17) 681 ± 141 (17) 241 ± 73 (15) 623 ± 179 (17)715 ± 205 Month 12  (17) 578 ± 147 (16) 654 ± 152 (16) 326 ± 67 (17) 819± 175 (16) 632 ± 155 p value 0.069 0.610 0.955 0.211 0.501

What is claimed is:
 1. A method for treating a fibrosing disease in apatient, comprising orally administering to the patient one or morecollagen fragments, said collagen fragments selected from the groupconsisting of: α1(I) CB1, CB2, CB3, CB4, CB5, CB6, CB7, CB8 and α2(I)CB1, CB2, CB3, CB4 and CB5.
 2. The method of claim 1, wherein saidfibrosing disease is selected from the group consisting of skinfibrosis, liver cirrhosis, renal fibrosis, lung fibrosis, heartfibrosis, gastrointestinal fibrosis and vascular fibrosis.
 3. The methodof claim 1, wherein said patient has been suffering from said fibrosingdisease for at least 3 years.
 4. The method of claim 1, wherein saidcollagen is derived from human or an animal species other than human. 5.The method of claim 1, wherein said collagen is orally administered tosaid patient at about 500 μg/day.
 6. The method of claim 1, wherein thepatient is treated for about 12 months.
 7. The method of claim 1,wherein the patient is treated for about 36 months.
 8. The method ofclaim 1, wherein the oral administration of said collagen inducestolerance in said patient.
 9. A dosage form for oral administration fortreating a fibrosing disease in a patient, comprising one or morecollagen fragments, said collagen fragments selected from the groupconsisting of: α(I) CB2, CB3, CB4, CB5, CB6, CB7, CB8, and α2(I) CB0,CB1, CB2, CB3, CB4 and CB5.
 10. The dosage form of claim 9, wherein saiddosage form is adapted for daily administration.
 11. The dosage form ofclaim 9, wherein said one or more collagen fragments is adapted toinduced tolerance in said patient upon oral administration.
 12. Thedosage form of claim 9, wherein said dosage form is orally administeredto a patient at about 500 μg/day.